Yıl 2015,
Cilt: 3 Sayı: 1, 334 - 339, 01.01.2015
Öz
Karbonik anhidraz enzimi (EC: 4.2.1.1; CA) sığır karaciğerinden saflaştırılarak bazı antibiyotiklerin inhibisyon etkileri incelendi. Saflaştırma prosedürü, homojenatın hazırlanması ve Sepharoz 4B-Tirozin-Sulfanamid Kromatografisinden oluştu. Birbirini takip eden bu iki yöntem ile
enzim 65,24 kat, % 50,86 verimle ve spesifik aktivitesi 1117.24 U/mg protein olarak saflaştırıldı. Enzim üzerine bazı antibiyotik ilaçların in vitro inhibisyon etkileri belirlendi. Bu inhibitörler için IC 50 değerleri, % aktiviteye karşı inhibitor konsantrasyon [I] grafiği çizilmek suretiyle belirlendi. Siprofloksazin ve seftazidim inhibitörlerinin IC değerleri sırasıyla 1.27 ve 5.29 mM olarak tespit 50 edildi.
Anahtar Kelimeler: Karbonik anhidraz(CA), Sığır, Karaciğer, İnhibitör, Antibiyotik
Anahtar Kelimeler
Kaynakça
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- carbonic anhydrase isoforms I, II, III, IV and VI. European Journal of Medicinal Chemistry. 49, 68-73, 2012.
- Demirdag, R., Yerlikaya, E., Senturk, M., Kufrevioglu, O.I., Supuran, C.T. Heavy metal ion inhibition studies of human, sheep and fish α-carbonic anhydrases. Journal of Enzyme Inhibition and Medicinal Chemistry. 28, 278-282, 2013.
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Toplam 33 adet kaynakça vardır.
Ayrıntılar
| Birincil Dil | İngilizce |
|---|---|
| Bölüm | Araştırma Makalesi |
| Yazarlar | |
| Yayımlanma Tarihi | 1 Ocak 2015 |
| Yayımlandığı Sayı | Yıl 2015 Cilt: 3 Sayı: 1 |